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Yes.

Without a GUI you will obviously not be able to use the graphical tools from ROS, such as Rviz, and RQT, but you will be able to use all of the core features such as publish-subscribe, services, roslaunch, and the command-line tools.

You can also run the ROS graphical tools on a laptop and desktop with a display, and connect them to your CLI computer over a wired or wireless network.